Advanced Photon Source

An Office of Science National User Facility

Regulated Soil

What to do if you want to analyze regulated foreign or domestic soil at the APS

Soil from foreign sources and certain locations in the United States are regulated in their movement into and about the country. A permit from the United States Department of Agriculture (USDA) is required to receive these soils unless they are sterilized. More complete information about the regulation of soil can be found on the USDA Soil Permit web page. More general information is available on the USDA APHIS website. Please contact Bruce Glagola well in advance of your experiment start date to arrange for use of regulated soil samples.

A few important concepts from the USDA Circular Q-330.300-2, Soil (01/2001) are:

  1. Why is soil regulated?
    Soil can contain numerous diseases and pests such as: animal and plant viruses, bacteria, fungi, nematodes, noxious weeds, and the life stages of destructive insects. In addition, adequate screening soil for the spectrum of organisms which might be harmful is not possible. Therefore, soil from all foreign countries and from US Territories and some states of the U.S. can move only if specific conditions and safeguards prescribed by the U.S. Department of Agriculture's, Animal and Plant Health Inspection Service (APHIS) are met. 7 CFR 330.300, 318.60, and the Plant Protection Act of 2000 as the authority lists the federal regulations for these conditions and safeguards. APHIS regulations protect U.S. agriculture from the introduction of destructive plant and animal diseases and pests."
  2. What is soil?
    Soil is the loose surface material of the earth in which plants grow in most cases consisting of disintegrated rock with mixture of organic material. This mixture can support biological activity and therefore carry and introduce harmful pests or diseases. Examples of soil are: topsoil, forest litter, wood or plant compost, humus, and earthworm castings."
  3. What is not soil?
    Materials free of organic matter, such as: pure sand, clay, talc, rocks, volcanic pumice, chalk, salt, diatomaceous earth, iron ore and gravel. These materials must be mined or collected so they are free of organic material, such as roots, grasses, or leaf litter.

    Fertilizers that contain minerals, bone meal, and crushed grain are not soil. However grains in the mix may be regulated if they lack processing that prevents sprouting. Authorities for regulating imported grains are — corn relatives (CFR 319.24 & 41), rice relatives (CFR 319.55) and wheat relatives (CFR 319.59).

    What has or may have organic matter in it and may be considered soil, but PPQ has determined is exempt from USDA soil regulations?

    The following items are exempt:
    • Peat, cosmetic mud, and other mud products from fresh water or the earth's upper surface, if processed to a uniform consistency, and free of plant parts or seeds.
    • Volcanic rock, pumice, geological samples, drilling cores, or mud, if mined so it is free of organic material.
    • Any sediment, muds, or rock from the saltwater oceans of the earth.

    NOTE: All shipments may be inspected at a U.S. port to identify the material and verify it is free of pests and prohibited contaminants. A shipment may be refused entry or require treatment based upon inspection findings."
  4. What U.S. soils are regulated:
    In general, soils from the southeast United States are regulated for movement. Soil from some other locations are also regulated. See the map provided by APHIS for locations of regulated domestic soil (US Soil Map).For details of other areas that may be regulated please consult the APHIS website listed above.
Does the APS have a permit to receive regulated foreign and domestic soil samples?

Yes, the APS has received a USDA permit to receive and manage regulated soil samples. The permit is #P330-14-00367.

What if I need help deciding if my samples are regulated soil?

If you are having difficulty deciding if your samples are "soil" or if they are regulated please contact Bruce Glagola (630/252-9797,

What do I have to do to ship a regulated soil sample to the APS?

Contact the APS to arrange for the shipment. The following criteria must be met for soil sample shipments that are subject to the USDA soil permit regulations:

  • All shipments of regulated soil must be less than three (3) pounds in total weight.
  • The soil must be double bagged and packaged in sturdy, leak-proof containers.
  • The shipment must be accompanied with a current copy of the APS soil permit.Place the permit inside the package.A copy of the current APS Soil Import Permit can be downloaded here. This is a new permit issued in January 2009. The old permit can no longer be used.
  • The package must be labeled using USDA PPQ Form 550 (Oct 2010) that is barcoded and numbered. Labels can be obtained by contacting Patti Pedergnana or Bruce Glagola. NOTE: Please contact us enough in advance to mail the labels to you. The USDA requires an original of the label.
  • The package must be shipped by bonded carrier (such as FedEx) from the port of arrival to the following address:

Patti Pedergnana
Bldg 431Z020
Argonne National Laboratory
9700 S. Cass Ave.
Argonne, IL 60439
United States

What will happen to my soil sample when it arrives at the APS?

All regulated soil samples will be stored in a locked cabinet in Room 431Z020 until needed at the beamline. All samples are entered into a logbook with the following information:

  • Date of sample arrival at the APS
  • Beamline where sample will be used
  • Weight of the sample
  • General composition of the sample
  • Origin of each incoming soil sample
  • User responsible for the sample
  • Date and location of sample transfer for analysis
  • Final disposition of the sample after the experiment.

Contact the Floor Coordinators Patti Pedergnana, Shane Flood, or Wendy VanWingeren to have your sample delivered to the beamline and for training on how the sample must be handled while at the APS.

When the soil sample is not being analyzed it must be secured (when unattended) at the beamline and the area posted for regulated soil samples.Secured is defined as in a locked location (i.e. a lockable drawer or safe).If the sample is left unattended while in the Experiment Station, the station doors must be secured with a chain and lock.Please contact the Floor Coordinator on duty for assistance.

How do I get my soil sample returned to my home institution after the experiment?

Regulated soil samples may only be received by someone that has a USDA permit. In order to ship a regulated soil sample out from the APS you must present a copy of the receiving institutions soil permit for inclusion in the package. The APS will also have to obtain written permission from an appropriate USDA PPQ Office to send the shipment.

How can I treat my sample so it may ship as unregulated soil?

In order to convert a soil sample from regulated to unregulated it must be heat treated according to USDA guidelines. Heat treating temperatures and times accepted by the USDA are listed in the APS Protocols for Handling, Storage, and Disposal of regulated soil

Can I dispose of my soil samples when the experiment is finished?

Instead of shipping a sample out from the APS it may be disposed of at the APS by having the soil sample heat treated according to USDA guidelines. Arrangements for disposal must be made with Patti Pedergnana, Wendy VanWingeren, or Bruce Glagola before you leave the APS.

How do I treat laboratory equipment that comes in contact with the regulated soil sample?

Any piece of laboratory equipment that comes in contact with an untreated (i.e unsterilized) regulated soil sample must be washed with a 70% alcohol solution. This may be done in the LOM laboratory where sample preparation is being conducted.

Updated: March 23, 2017